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      sticky-Slide Chemotaxis

      Model:80328 10812

      Brand:ibidi

      Specifications :10 (individually packed)

      Introduction:A chemotaxis bottomless slide used in 2D and 3D applications with a self-adhesive underside designed to allow self-assemblage of the slide setup Versatile – i.e., permits the mounting of a variety of bottom materials

      Applications:

      Inserting materials or tissue into chemotaxis chambers

      Keeping cell spheroids or tissue in a stable concentration gradient

      Chemotaxis of fast or slow migrating cells on either a 2D surface or in 3D gels

      Usable with a range of bottom materials, such as plastic sheets, silicon chips, and glass slides

      Specifications:

      Outer dimensions (w x l)25.5 x 75.5 mm2
      Chemotaxis chambers on slide3
      Volume per chamber130 μl
      Observation area2 x 1 mm2
      Height of reservoirs1.05 mm
      Total height with plugs12 mm
      Volume chemoattractant30 μl
      Bottomnone


      Technical Features:

      Bottomless slide

      Self-adhesive underside

      Biocompatible adhesive that has been cell culture tested

      Adheres to all flat surfaces, even wet surfaces

      Suitable coverslips available

      Specifications identical to those of the μ-Slide Chemotaxis, except that it has no bottom

      Placing Tissue Sample in the sticky-Slide Chemotaxis

      Application Example: Directed Invasion of MCF-7 Cancer Cells in an FCS Gradient

      This movie shows the invasion of MCF-7 cancer cells into a collagen gel during 48 hours. The center channel of a sticky-Slide Chemotaxis was increased in height by milling in order to position the MCF-7 spheroid. The left side contains 10% fetal calf serum, the right side is serum-free. Phase contrast microscopy, objective lens 4x.

      Technical Drawing

      Technical drawings and details are available in the Instructions (PDF).

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